1. The technique electrophoresis, for the separation of charged molecules was developed by
a) Tswett
b) Svedberg
c) Tiselius
d) Sanger
Answer: c) Tiselius
2. In electrophoresis, DNA will migrate towards
a) cathode or positive electrode
b) anode or negative electrode
c) cathode or negative electrode
d) anode or positive electrode
Answer: d) anode or positive electrode
3. The speed of migration of ions in an electric field depends on
a) magnitude of charge and mass of molecules
b) magnitude of charge and shape of molecules
c) shape and size of the molecule
d) magnitude of charge, shape and mass of molecules
Answer: b) magnitude of charge and shape of molecules
4. Which of the following statement is true regarding migration of bio molecules?
a) the rate of migration is directly proportional to the current
b) the rate of migration is inversely proportional to current
c) the rate of migration is directly proportional to the resistance of the medium
d) Low voltage is used for the separation of high molecular weight compounds
Answer: a) the rate of migration is directly proportional to current
5. Electrophoresis cell or electrophoresis apparatus consists of
a) power pack and electrophoresis unit
b) Electrophoresis unit and DNA separator
c) buffer chamber and Electrophoresis unit
d) Gel, buffer chamber and power pack
Answer: a) power pack and electrophoresis unit
6. The most common type of gel used for DNA separation is
a) Agar
b) Polyacrylamide
c) Agarose
d) All of the above
Answer: c) Agarose
7. Which is the technique suited for the separation of large DNA fragments
a) AGE
b) PAGE
c) PFGE
d) SDS-PAGE
Answer: c) PFGE
8. What is the role of SDS in SDS-PAGE?
a) protein denaturing and imparting net negative charge
b) imparting overall negative charge to the protein
c) imparting equal mass to all proteins
d) protein unfolding and imparting net positive charge
Answer: a) protein denaturing and imparting net negative charge
9. In SDS-PAGE, separation is based on
a) molecular weight
b) shape
c) charge
d) all of the above
Answer: a) molecular weight
10. The electrophoresis technique that used isoelectric focusing is
a) AGE
b) PFGE
c) 2D-PAGE
d) SDS-PAGE
Answer: c) 2D-PAGE
5 Types of Electrophoresis
1. SDS-PAGE
2. Native-PAGE
3. Isoelectric Focusing
4. Capillary Electrophoresis
5. Band Detection
a) Tswett
b) Svedberg
c) Tiselius
d) Sanger
Answer: c) Tiselius
2. In electrophoresis, DNA will migrate towards
a) cathode or positive electrode
b) anode or negative electrode
c) cathode or negative electrode
d) anode or positive electrode
Answer: d) anode or positive electrode
3. The speed of migration of ions in an electric field depends on
a) magnitude of charge and mass of molecules
b) magnitude of charge and shape of molecules
c) shape and size of the molecule
d) magnitude of charge, shape and mass of molecules
Answer: b) magnitude of charge and shape of molecules
4. Which of the following statement is true regarding migration of bio molecules?
a) the rate of migration is directly proportional to the current
b) the rate of migration is inversely proportional to current
c) the rate of migration is directly proportional to the resistance of the medium
d) Low voltage is used for the separation of high molecular weight compounds
Answer: a) the rate of migration is directly proportional to current
5. Electrophoresis cell or electrophoresis apparatus consists of
a) power pack and electrophoresis unit
b) Electrophoresis unit and DNA separator
c) buffer chamber and Electrophoresis unit
d) Gel, buffer chamber and power pack
Answer: a) power pack and electrophoresis unit
6. The most common type of gel used for DNA separation is
a) Agar
b) Polyacrylamide
c) Agarose
d) All of the above
Answer: c) Agarose
7. Which is the technique suited for the separation of large DNA fragments
a) AGE
b) PAGE
c) PFGE
d) SDS-PAGE
Answer: c) PFGE
8. What is the role of SDS in SDS-PAGE?
a) protein denaturing and imparting net negative charge
b) imparting overall negative charge to the protein
c) imparting equal mass to all proteins
d) protein unfolding and imparting net positive charge
Answer: a) protein denaturing and imparting net negative charge
9. In SDS-PAGE, separation is based on
a) molecular weight
b) shape
c) charge
d) all of the above
Answer: a) molecular weight
10. The electrophoresis technique that used isoelectric focusing is
a) AGE
b) PFGE
c) 2D-PAGE
d) SDS-PAGE
Answer: c) 2D-PAGE
5 Types of Electrophoresis
1. SDS-PAGE
2. Native-PAGE
3. Isoelectric Focusing
4. Capillary Electrophoresis
5. Band Detection
If the answers is incorrect or not given, you can answer the above question in the comment box. If the answers is incorrect or not given, you can answer the above question in the comment box.